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KMID : 0364020100430040394
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Korean Journal of Thoracic and Cardiovascular Surgery
2010 Volume.43 No. 4 p.394 ~ p.398
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The Correlation between TGF-beta 1 Blood Levels and the Formation of Bullae in Patients with Spontaneous Pneumothorax
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Kim Young-Sam
Kim Kwang-Ho
Baek Wan-Ki
Kim Joung-Taek
Kim Ji-Hye
Cha Il-Kyu
Song Sun-U.
Choi Mi-Sook
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Abstract
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Background: The overexpression of transforming growth factor-beta 1 receptor II (TGF-?1RII) and transforming growth factor-beta 1 (TGF-?1) ligand may be involved in the formation of a bulla. In this study, we tested if serum TGF-?1 ligand levels correlated with the expression level of TGF-?1RII and TGF-?1 in bullous tissues from patients with spontaneous pneumothorax.
Material and Method: Bullous lung tissues and blood samples were obtained from 19 patients with spontaneous pneumothorax, 18 males and 1 female, aged 17 to 35 years old. The bullous tissues were obtained by video-assisted thoracic surgery (VATS), fixed in formalin, embedded in paraffin, and cut into 5∼6?m thick slices. Sections were immunohistochemically stained with primary antibodies against TGF-?1 or TGF-?1RII, and serum levels of TGF-?1 in patients and normal controls was measured by enzyme-linked immunosorbent assay (ELISA).
Result: Of the 19 patients, 16 were TGF-?1 positive and 10 were TGF-?1RII positive. Among the 16 TGF-?1 positives, 9 were also TGF-?1RII positive. As seen previously, strong immunohistochemical staining of TGF-?1RII and TGF-? was detected in the boundary region between the bullous and normal lung tissues. Average TGF-?1 blood levels of both TGF-?1 and TGF-?1RII positive patients was 38.36±16.2 ng/mL, and that of five controls was 54.06±15 ng/mL.
Conclusion: These results suggest that overexpression of TGF-?1 and TGF-?1RII expression may be involved in the formation of bullae. TGF-?1 blood levels in patients with primary spontaneous pneumothorax is lower than normal people, suggesting that the high level of local TGF-?1 expression in the bullous tissue region, but not in the whole blood, may contribute more in the formation of bullae. (Korean J Thorac Cardiovasc Surg 2010;43:394-398)
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KEYWORD
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Growth factor, Bulla, Pneumothorax
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